Fig. 6
NLK mediates the degradation of MAVS. a NLK-induced dose-dependent degradation of MAVS. HEK293T cells were transfected with HA-MAVS and increasing amounts of Myc-NLK. The protein levels of MAVS and NLK were determined after transfection for 12 h through immunoblotting using the HA and Myc antibodies. b, c NLK and NLKKM were analyzed for MAVS degradation. b HEK293T cells were transfected with HA-MAVS and Flag-NLK or Flag-NLKKM. The protein levels of MAVS and NLK were determined via immunoblot analysis using HA and Flag antibodies. c HEK293T cells were transfected with Myc-NLK or Myc-NLKKM. The protein levels of MAVS and NLK were determined through immunoblot analysis using MAVS and Myc antibodies. d Stability of MAVS in wild-type and NLK-deficient BMDMs. BMDMs were treated with CHX for the indicated periods, and the cell lysate was then subjected to immunoblot analysis using the MAVS antibody. Relative levels of MAVS were calculated after normalization to GAPDH and are shown in the right panel. e Stability of MAVS in wild-type and NLK-deficient BMDMs after SeV infection. The BMDMs were treated with CHX for the indicated periods, and the cell lysate was then subjected to immunoblot analysis using MAVS antibodies. The relative levels of MAVS were calculated after normalization to GAPDH and are shown in the right panel. Data are representative of three independent experiments. Source data (a–e) are provided as a Source Data file
