Fig. 3
Comparison of ALB+ cells in human liver scRNA-seq populations. a 2D t-SNE visualisation of ALB+ cells isolated from foetal and adult human liver coloured by tissue type (left panel) and K-means cluster (right panel). Phenotypic labelling based on transcriptional analysis. b Comparison of foetal and adult hepatobiliary hybrid progenitors (HHyP) significantly enriched genes (FC 1.1, p-val < 0.05 with student t test) by venn diagram with top ten highly expressed genes in foetal (left) and adult (right) HHyPs. c Gene set enrichment analysis (GSEA) of foetal vs. adult HHyPs for Gene ontology (GO) terms ‘Stem cell proliferation’, ‘Developmental cell growth’, ‘Homophilic cell adhesion via plasma membrane adhesion molecules’ and ‘Extracellular matrix component’. d Transcript expression of selected markers overlaid on the 2D t-SNE space of human liver scRNA-seq analysis for adult HHyP-restricted genes (top), foetal HHyP-restricted genes (middle) and other cell type-specific expression patterns (bottom). Expression is Log10(TPM). e Heatmap of top foetal HHyP up regulated genes in publicly available sequencing data from human hepatic liver progenitor cells (hepLPCs-Heps, GSE105019) converted from primary hepatocytes16. Heatmap shows expression in primary hepatocytes (pH) and human primary hepatocytes converted into liver progenitor-like cells (HepLPCs) at different stages in transition and expansion medium (TEM). The colour bar indicates gene expression in log10 scale. t-SNE t-distributed stochastic neighbour embedding, TPM transcripts per million, FC fold change
