Fig. 4
Nogo-B promotes lipid droplet degradation in HCC cells. a Immunohistochemical staining of Nogo-B and Nile red staining of murine NASH-associated HCCs. b Co-localization of GFP-Nogo-B and LDs in LO2 cells transfected with Nogo-B for 48 h and incubated with oxLDL for 24 h followed by starvation. c Representative LipidTOX staining (top) and quantification of positively stained areas (bottom) of Nogo-B-overexpressing LO2 cells treated with oxLDL for 24 h followed by starvation in 0.1% FBS for 12 h. Cellular lipid droplets were stained using LipidTOX for 30 min. d Representative LipidTOX staining (top) and quantification of positively stained areas (bottom) of oxLDL-loaded MHCC97H cells stably transfected with shCtrl or shNogo-B with starvation or without starvation (resting) in 0.1% FBS for 12 h. e, f Representative LipidTOX staining (top) and quantification of positively stained areas (bottom) of e Nogo-B-overexpressing LO2 cells and f MHCC97H cells stably transfected with shCtrl or shNogo-B and treated with oxLDL for 24 h. g, h FFA concentration in supernatant (left) and TG concentration in cell lysate (right) extracted from g Nogo-B-overexpressing LO2 cells and h MHCC97H cells stably transfected with shCtrl or shNogo-B and treated with oxLDL for 24 h followed by starvation. i The domain organization and dissection of the human Nogo-B protein. j Representative LipidTOX staining (left) and quantification of positively stained areas (right) of wile-type Nogo-B (WT) or ER-motif-deficient Nogo-B (d38) ectopically expressed LO2 cells treated with oxLDL for 24 h followed by starvation in 0.1% FBS for 12 h. (Data are presented as mean ± SEM of three independent experiments in c–h and j. *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file)
