Fig. 6
Nogo-B stimulates the Hippo pathway in HCC cells. a Pathway reporter luciferase array revealed signaling deregulation by Nogo-B in LO2 cells. Cells were transfected with different pathway luciferase reporters for 48 h. b Heatmap of lipidomics signatures in livers from HFHC-promoted HCCs (n = 2 in each group). c Western blot (top) and qRT-PCR (bottom) analyses of YAP activity in LO2 cells upon ectopic expression (left) and MHCC97H cells upon knockdown (right) of Nogo-B. d LPA concentrations in the supernatant (top) and LPC concentrations in the cell lysate (bottom) of LO2 cells upon Nogo-B ectopic expression (left) and MHCC97H cells upon Nogo-B knockdown (right). Stable cell lines were treated with oxLDL for 24 h followed by starvation. Supernatant and cell lysate were collected for detection. e Western blot analysis of p-YAP and YAP in control or Nogo-B-overexpressing LO2 cells transfected with ATG5 siRNAs for 48 h and treated with oxLDL for 24 h followed by starvation. f LPA concentrations in the supernatant of control or Nogo-B-overexpressing LO2 cells transfected with ATG5 siRNAs for 48 h and treated with oxLDL for 24 h followed by starvation. g Western blot analysis of p-YAP and YAP in control or Nogo-B-overexpressing LO2 cells with treatment of oxLDL for 6 h and then additional PF-8380 (250 nM) treatment for 36 h followed by starvation. h Western blot analysis of p-Yap and Yap in paired tumors (T) and normal tissues (NT) from HFHC-fed mice (n = 3 in each group). i Western blot analysis of p-Yap and Yap of the liver tissues from HFHC-fed mice administered with lentivirus expressing shCtrl or shNogo-B (n = 4 in each group). j 22:6 LPA concentrations in the liver tissues from HFHC-fed mice administered with lentivirus expressing shCtrl (n = 8) or shNogo-B (n = 10). k Correlation analysis of 22:6 LPA concentrations and Nogo-B expression levels in the liver tissues from HFHC-fed mice administered with lentivirus expressing shCtrl (n = 8) or shNogo-B (n = 10). (Data are presented as mean ± SEM of three independent experiments in a, c, d, and f. *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file)
