Fig. 5

Stroma ZEB1-deleted mammary glands exhibit a suppressive tumour microenvironment phenotype. a In total, 2 × 10⁶ PyMT-cancer cells were orthotopically transplanted into Fib-WT and -cKO mammary glands (five mice, each), and tumour volume monitored 2 weeks and 4 weeks after transplantation (n = 5 independent experiments). Two-sided Student’s t test. b Haematoxylin and eosin (H&E) and Masson’s staining of orthotopic tumours as described in a (images are representative of images from five mice). Magnified areas of boxed sections are shown in middle panels. LN lymph node. c Immunofluorescence staining of CD31, VEGFA, CD45 and F4/80 in the indicated orthotopic tumours as described in a (images are representative of images from five mice). Nuclei are DAPI stained. d Quantification of relative fluorescent intensities in ten random fields of each section of the indicated orthotopic tumours as shown in c (n = 5 independent experiments). Two-sided Student’s t test. e Immunohistochemical staining of Ki67 and phospho-histone H3 in the indicated orthotopic tumours as described in a (images are representative of images from five mice). f Quantification of Ki67- and phospho-histone H3 (p-H3)-positive cells as shown in e. For quantification, around 1000 cells are counted in ten random fields of each section (n = 5 independent experiments). Two-sided Student’s t test. g K14 immunofluorescent staining of the indicated orthotopic tumours from the indicated mice, as described in a (images are representative of images from five mice). Nuclei are DAPI stained. The arrows denote strands of K14⁺ invading leader cells at the invasive borders. All data are represented as mean ± s.d. **P < 0.01. The source data are provided as a Source Data file