Fig. 6
From: Smu1 and RED are required for activation of spliceosomal B complexes assembled on short introns
Cleavage of the MINX80 intron restores spliceosome activation in ΔSmu1/RED nuclear extract. a Schematic of the MINX80 and cut-MINX80 pre-mRNAs. Cut-MINX80 was generated by transcribing the MINX80 pre-mRNA in two parts, where only the 5′ half was 32P-radiolabelled (indicated in red). b, c Kinetics of in vitro splicing (b) and spliceosome assembly (c) of the MINX80 and cut-MINX80 pre-mRNAs in mock- and Smu1/RED-depleted extract. The pre-mRNA and splicing intermediates/products, as well as spliceosomal complex formation, were analysed as in Fig. 2. Source data are provided as a Source Data file
