Fig. 3
From: Standard screening methods underreport AAV-mediated transduction and gene editing
AAV8 transduces kidney glomeruli. a Representative fluorescent photomicrographs of kidney sections from C57BL/6J and Ai14 mice 2 weeks after IV delivery of AAV8-Cre-eGFP at the indicated doses. No eGFP expression is detected in green channel in either mouse strain for all doses tested. tdTomato (red) indicates editing; nuclei are shown (blue). n = 2–3 for C57BL/6J mice, n = 3 for Ai14 mice. Scale bars = 250 µm. b Comparison of AAV vector copies per diploid genome equivalent (dge) in kidney samples from C57BL/6J and Ai14 mice 2 weeks post IV delivery. Values for individual mice are shown along with the mean and standard error for each strain and dose group. c Cre-mediated recombination at the ROSA26 locus as detected by PCR assay of kidney genomic DNA from Ai14 mice. d Immunohistochemistry for glomerular cell types in Ai14 mice 2 weeks after AAV8-Cre-eGFP delivery (3.16e11 vg). Primary antibodies labeled podocytes (anti-podoplanin), endothelial cells (anti-CD31), and mesangial cells (anti-PDGFR-β) and were detected with Alexa-488 conjugated secondary antibodies (green). Cre-tagged cells are tdTomato positive. Scale bars = 25 µm. Source data are provided as a Source Data file
