Fig. 1
Phosphatidic acid (PA) in early Coat Protein I (COPI) vesicle fission. Quantitative data are shown as mean ± s.e.m. Significance was tested using the two-tailed Student’s t test, **P < 0.01, ***P < 0.0001, NS (non-significant) P > 0.05. a Golgi membrane with reduced lysophosphatidic acid acyltransferase type gamma (LPAAT−γ) level was used for the COPI vesicle reconstitution system. Rescue used PA forms as indicated; n = 5 independent experiments. b Golgi membrane with reduced phospholipase D type 2 (PLD2) level was used for the COPI vesicle reconstitution system. Rescue used PA forms as indicated; n = 5 independent experiments. c HeLa cells were treated with small interfering RNA (siRNA) against LPAAT−γ. Rescue used PA forms as indicated. COPI transport in cells was tracked by examining the quantitative colocalization of a COPI-dependent cargo protein (VSVG-KDELR) with Golgi marker (giantin) at different time points as indicated, n = 4 independent experiments. d HeLa cells were treated with siRNA against PLD2. Rescue used PA forms as indicated. COPI transport in cells was tracked by examining the quantitative colocalization of a COPI-dependent cargo protein (VSVG-KDELR) with Golgi marker (giantin) at different time points as indicated, n = 4 independent experiments. e Golgi membrane with reduced LPAAT−γ level was used for the COPI vesicle reconstitution system. Rescue used PA forms as indicated; n = 5 independent experiments. f Golgi membrane with reduced PLD2 level was used for the COPI vesicle reconstitution system. Rescue used PA forms as indicated; n = 5 independent experiments. g HeLa cells were treated with siRNA against LPAAT−γ. Rescue used PA forms as indicated. COPI transport in cells was tracked by examining the quantitative colocalization of a COPI-dependent cargo protein (VSVG-KDELR) with Golgi marker (giantin) at different time points as indicated, n = 4 independent experiments. h HeLa cells were treated with siRNA against PLD2. Rescue used PA forms as indicated. COPI transport in cells was tracked by examining the quantitative colocalization of a COPI-dependent cargo protein (VSVG-KDELR) with Golgi marker (giantin) at different time points as indicated, n = 4 independent experiments. Source data are provided as a Source Data file
