Fig. 1
From: Mitofusins regulate lipid metabolism to mediate the development of lung fibrosis
Altered mitochondrial dynamics in murine AEC2 cells in bleomycin-induced lung fibrosis. a Schema demonstrating the generation of mice with tamoxifen-inducible tdTomato labeling in AEC2 cells; tdTomato reporter mice (Rosa26tdTomato+/+) were crossed with SftpcCreERT2+/+ mice. b A functional enrichment map generated using genes differently expressed between AEC2 cells with and those without bleomycin treatment, using the threshold of an adjusted p < 0.001 and a fold change >1.2. c Heatmap showing upregulated genes under the annotation mitochondrial organization (GO:0007005) in AEC2 cells treated with bleomycin (BLM), compared to those treated with PBS. d Expression of Mfn1, Mfn2 and Dnm1l mRNA in AEC2 cells 5 days after PBS (n = 4 mice) or BLM (n = 4 mice) treatment. For each gene, the fold change of FPKM is calculated relative to the PBS group. The data are presented as mean±s.e.m. (NS, not significant; ***adjusted p < 0.001 vs. PBS group). e A heatmap showing downregulated genes under the annotation mitochondrial organization (GO:0007005) in AEC2 cells treated with bleomycin, compared to those treated with PBS. f Representative TEM images (50,000X) showing mitochondrial damage in AEC2 cells from SftpcCreERT2+/− mice before and after bleomycin treatment (scale bar 500 nm). g-i Representative TEM images (12,000X; scale bar 2 μm) (g) and corresponding quantification of mitochondrial number per μm2 of cytosolic area (h) and mitochondrial area (μm2) (i) in each AEC2 cell before and after bleomycin treatment. Each dot represents one AEC2 cell and the line indicates mean (before bleomycin, n = 15 AEC2 cells from 3 mice; after bleomycin, n = 26 AEC2 cells from 2 mice; *p < 0.05, ***p < 0.001, vs. no bleomycin treatment by unpaired Student’s t test). Source data (c, d) are provided as a Source Data file
