Fig. 5
Coordination of glucose and amino acids catabolism. a Mean ± standard deviation of Crp activity across different nutritional environments: minimal medium with either glucose (GLC) or succinate (SUC in green, positive control), and glucose minimal media supplemented with casamino acids (CAA), synthetic amino acid mix (SAA), SAA deprived of amino acids that can be degraded into pyruvate: (i) threonine, glycine and serine (STG) or (ii) threonine, glycine, serine, tryptophan, cysteine, and alanine (STGTCA), or α-ketoglutarate: (iii) glutamate, glutamine, proline, and arginine (GGPA), or oxaloacetate: (iv) aspartate and asparagine (AA), and glucose minimal medium with 0.125 g/L of glutamate (GLT). b Dynamic changes of the ratio between phosphoenolpyruvate and pyruvate upon CAA supplementation. c Dynamic relative changes in the abundance of pyruvate (blue), oxaloacetate (red), and α-ketoglutarate (yellow) upon supplementation with the different amino acid mixtures and glucose as the main carbon source. d Relative changes in the abundance of pyruvate 30 min after amino acid addition vs. the ratio between acetate secretion and glucose consumption. e In vitro activity of PtsI in the presence of only the reactant phosphoenolpyruvate (PEP), or PEP with the addition of glutamate (GLT), alanine (ALA), aspartate (ASP), fructose bis-phosphate (FBP), succinate (SUC), glyoxylate (GOX), α-ketoglutarate (AKG), oxaloacetate (OXA), and oxamate (OXM). Bar plots report mean ± standard deviation across three replicates
