Fig. 2

Inward-open structure of ASCT2_C467R. Cryo-EM map of ASCT2_C467R obtained in presence of TBOA at 3.6 Å resolution (a) and respective model shown as ribbon (b). c Protomer of ASCT2 with labelled structural elements is shown as ribbon, rotations are indicated. The scaffold domains are coloured in yellow, the extracellular antenna in red, the transport domains in blue, the HP2 loop in purple, and non-protein densities in grey (see also Supplementary Fig. 7). The membrane boundary is indicated and the location of the substrate binding site as shown in Fig. 3 is highlighted by a black rectangle in panel b