Table 1 Cryo-EM data collection, refinement and validation statistics
From: A one-gate elevator mechanism for the human neutral amino acid transporter ASCT2
|  | ASCT2C467R—TBOA (EMD-10016, PDB 6RVX) | substrate-free ASCT2C467R (EMD-10018, PDB 6RVY) | substrate-free ASCT2wt (EMD-10017) |
|---|---|---|---|
Data collection and processing | |||
 Magnification | 49,407 | 49,407 | 49,407 |
 Voltage (kV) | 200 | 200 | 200 |
 Electron exposure (e−/Å2) | 52 | 52 | 52 |
 Defocus range (μm) | −0.8 to −1.8 | −0.8 to −1.8 | −0.8 to −1.8 |
 Pixel size (Å) | 1.012 | 1.012 | 1.012 |
 Symmetry imposed | C3 | C3 | C3 |
 Initial particle images (no.) | 987,852 | 245,367 | 317,611 |
 Final particle images (no.) | 223,354 | 23,664 | 19,651 |
 Map resolution (Å) | 3.61 | 4.13 | 6.98 |
 FSC threshold | 0.143 | 0.143 | 0.143 |
 Map local resolution range (Å) | 4.2–3.4 | 6.4–4.1 | 8.0–6.3 |
Refinement | |||
 Initial model used | PDB 6GCT | PDB 6GCT | – |
 Model resolution (Å) | 3.6 | 4.1 | – |
(0.5 FSC threshold) | Â | Â | Â |
 Model resolution range (Å) | 15–3.6 | 15–4.1 |  |
 Map sharpening B factor (Å2) | −205 | −211 | −764 |
Model composition | |||
  Nonhydrogen atoms | 9627 | 9627 | – |
  Protein residues | 1290 | 1290 | – |
  Ligands | 0 | 0 | – |
B factors (Ã…2) | |||
  Protein | 19.03 | 83.05 | – |
  Ligand | – | – | – |
R.m.s. deviations | |||
  Bond lengths (Å) | 0.006 | 0.006 | – |
  Bond angles (°) | 0.979 | 1.023 | – |
Validation | |||
  MolProbity score | 1.75 | 1.84 | – |
  Clashscore | 5.00 | 6.72 | – |
  Poor rotamers (%) | 0.29 | 0.49 | – |
Ramachandran plot | |||
  Favoured (%) | 92.02 | 92.49 | – |
  Allowed (%) | 7.98 | 7.51 | – |
  Disallowed (%) | 0.00 | 0.00 | – |
