Fig. 6 | Nature Communications

Fig. 6

From: Camouflaging bacteria by wrapping with cell membranes

Fig. 6

Analysis of bacterial decoating. a–c Assays for the stability of the coating membranes. The ratios of CMCB to uncoated bacteria were measured by flow cytometry at various time points after CMCB were incubated in a 100% serum and LB medium at 37 °C, b ice-cold PBS, and c 100% serum with 5 μg/ml of SMX. Error bars represent the standard deviation (n = 3). d Representative LSCM images of bacteria after culturing CMCB in PBS at 37 °C for 1 h. The red channel shows EcN expressing mCherry, the green channel shows cell membranes labelled with FITC-affinity anti-CD47-antibody, and the merge (light orange) shows CMCB. Scale bar, 10 μm. e A representative image of membrane shedding captured by TEM. Scale bar, 1 μm. f Schematic illustration for the removal of coating membranes

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