Fig. 6

Immune and lung clocks contribute to circadian gating of IAV infection. Experimental design: C57bl6 mice were maintained in reverse cycles of 12 h LD for 2 weeks. Thereafter, Nk1.1 antibody administered to deplete Nk1.1⁺ cells, and one day later the animals were infected with IAV (PR8) at ZT23 and ZT11. a Survival. b Weight change trajectory (N = 20–32/group form three independent experiments). Experimental design: Bmal1^fl/flLysMcre⁺ mice (mice lacking Bmal1 in the myeloid cells) and their Bmal1^+/+cre^+/+ littermates were acclimatized to reverse cycles of 12 h LD for 2 weeks. Thereafter, they were maintained in constant darkness for 1 week prior to administering IAV (PR8) at CT23 and CT11. c Survival. d Weight change trajectory (n = 11–15/group from three independent experiments). Experimental design: Bmal1^fl/flCCSPcre⁺ mice (mice lacking Bmal1 in club cells of the lung epithelium) and their cre⁻ littermates were acclimatized to reverse cycles of 12 h LD for 2 weeks. Thereafter, they were maintained in constant darkness for 1 week prior to administering IAV (PR8) at CT23 and CT11. e Survival (f) weight change trajectory (n = 9 in cre⁺ groups and n = 9–16 in the cre⁻ group in two independent experiments). Survival curves composite of 2–4 independent experiments (log-rank (Mantel–Cox) test, *p < 0.05). The data expressed as mean ± SEM in panels b, d, f. Source data are provided as a Source Data file