Fig. 2

RNA-seq analysis of EPCAM-positive endometrial epithelial cells isolated via magnetic sorting. a Schematic of EPCAM isolation using anti-EPCAM-PE antibody and anti-PE microbeads. b EPCAM is expressed in the endometrial epithelium of a LtfCre^0/+; (Gt)R26Pik3ca^*H1047R; Arid1a^fl/fl mouse by IHC (N = 3). Arrows indicate endometrial epithelium (scale bar = 100 μm). c IF staining of EPCAM and ARID1A in mouse endometrium (N ≥ 3). Arrows indicate endometrial epithelium (scale bar = 25 μm). d qPCR analysis of Arid1a gene expression of isolated control (N = 3, pooled groups of six mice) and LtfCre^0/+; (Gt)R26Pik3ca^*H1047R; Arid1a^fl/fl (mutant) (N = 4, single mice) cells (mean ± s.d; **p < 0.01, unpaired t-test, two-tailed). e, f Pathway enrichment analysis on human orthologs of differentially expressed genes between LtfCre^0/+; (Gt)R26Pik3ca^*H1047R; Arid1a^fl/fl, and control mice (FDR < 0.05; 3481 genes) for mSigDb Hallmark pathways (e) and Gene Ontology (GO) Biological Process terms (f). g GSEA plots showing significance of Mak et al. pan-cancer EMT signature upregulation within LtfCre^0/+; (Gt)R26Pik3ca^*H1047R; Arid1a^fl/fl compared to control and UCEC ARID1A^mut patients compared to ARID1A^wt. h Hierarchical clustering of 77 genes within the Mak et al. pan-cancer EMT signature between control and mutant purified endometrium. Genes found in the Hallmark EMT pathway, and CDH1, are identified