Fig. 6
PIK3CAH1047R antagonizes ARID1A loss-induced mesenchymal phenotypes. a Western blot of ARID1A, β-Actin, AKT, P-AKT, CDH1, SNAI1, SNAI2, and TWIST1 following co-transfection of siNONtg and empty vector (control), siARID1A and empty vector (siARID1A), siNONtg and pPIK3CAH1047R (PIK3CAH1047R), or siARID1A and pPIK3CAH1047R (siARID1A/PIK3CAH1047R). b Proportional Euler diagram displaying differentially expressed genes (FDR < 0.0001) from siARID1A, PIK3CAH1047R, and siARID1A/PIK3CAH1047R relative to control. c mSigDb Hallmark pathway enrichment for siARID1A, PIK3CAH1047R, and siARID1A/PIK3CAH1047R differentially expressed genes. d Enrichment for LtfCre0/+; (Gt)R26Pik3ca*H1047R; Arid1afl/fl mouse signature ortholog genes and Mak et al. pan-cancer gene signature within differentially expressed genes from siARID1A, PIK3CAH1047R, and siARID1A/PIK3CAH1047R relative to control. e, f Fold-change values of experimental groups relative to control for genes in the Mak and Tong pan-cancer EMT signature (e) and the Hallmark EMT signature (f), separated based on direction of gene expression change in siARID1A. Statistic represented is paired Mann–Whitney U (two-tailed). Box-and-whiskers plotted in the style of Tukey without outliers. g Intersection between siARID1A differentially expressed genes relative to control and siARID1A/PIK3CAH1047R relative to siARID1A. h Heat map detailing relative expression of intersecting genes (N = 127) (Fig. 6g) in control, siARID1A, PIK3CAH1047R, and siARID1A/PIK3CAH1047R, and ARID1A promoter binding. These genes were enriched for ARID1A promoter binding (p < 10−18, hypergeometric enrichment). i Expression level of intersect genes (Fig. 6g) in siARID1A, PIK3CAH1047R, and siARID1A/PIK3CAH1047R relative to control. Statistic represented is paired Mann–Whitney U (two-tailed). Box-and-whiskers plotted in the style of Tukey without outliers. j mSigDb Hallmark pathway enrichment for intersecting genes (N = 127) (Fig. 6g). k Changes in relative EMT gene expression upon ARID1A loss and PIK3CAH1047R overexpression as measured by qRT-PCR. Data represents three biological replicates
