Fig. 3
From: Maintenance of cell type-specific connectivity and circuit function requires Tao kinase
Tao kinase regulates postsynaptic growth of A08n neurons. a Confocal images of hemisegments in control or with TaoRNAi and TaoCA expression in A08n neurons using synaptic markers labeling of C4da presynapses (magenta) and A08n postsynapses (green). Scale bar = 5 µm. b Quantification of C4da presynaptic, c A08n postsynaptic, and d colocalized C4da–A08n synaptic markers in control or with TaoRNAi and TaoCA expression in A08n neurons. ***P < 0.001, **** P < 0.0001 ± SD, ANOVA with multiple comparisons and Dunnett’s post-hoc test (for exact P-values and statistics see Supplementary Data 1). Control n = 10, UAS-TaoRNAi n = 11, UAS-TaoCA n = 10. e Confocal images of Syb-GRASP-labeled C4da–A08n synapses. Hemisegments of control animals or with TaoRNAi and TaoCA expression in A08n neurons together with anti-Fas3 staining are shown. Fas3 labels C2da, C3da, and C4da sensory axons (blue) overlapping with reconstituted GFP signal within the C4da neuron domain (green). Scale bar = 5 µm. f Quantification of C4da–A08n neuron synapses using Syb-GRASP under control conditions or with TaoRNAi and TaoCA expression in A08n neurons. Control n = 9, UAS-TaoRNAi n = 7, UAS-TaoCA n = 10. *P < 0.05 ± SD, ANOVA with multiple comparisons and Dunnett’s post-hoc test (for exact P-values and statistics see Supplementary Data 1)
