Fig. 4
From: Maintenance of cell type-specific connectivity and circuit function requires Tao kinase
Tao restricts postsynaptic growth and connectivity during larval development. a–c Quantitative analysis of C4da–A08 neurons synaptic profiles from 48 h AEL to 120 h AEL in control or with TaoRNAi expression in A08n neurons. a C4da neuron presynapse, b A08n postsynapse, and c C4da–A08n synapse numbers. Control 48 h n = 10, 72 h n = 8, 96 h n = 9, 120 h n = 10. UAS-TaoRNAi 48 h n = 10, 72 h n = 9, 96 h n = 10, 120 h n = 8. d C4da–A08 synapse to C4da presynapse ratios (in percent) during development from 48 h AEL to 120 h AEL in control or with TaoRNAi expression in A08n neurons. Control 48 h n = 10, 72 h n = 8, 96 h n = 9, 120 h n = 10. UAS-TaoRNAi 48 h n = 10, 72 h n = 9, 96 h n = 10, 120 h n = 8. e C4da–A08n synapse to A08n postsynapse ratios (in percent) during development from 48 h AEL to 120 h AEL in control or with TaoRNAi expression in A08n neurons. Control 48 h n = 10, 72 h n = 8, 96 h n = 9, 120 h n = 10. UAS-TaoRNAi 48 h n = 10, 72 h n = 9, 96 h n = 10, 120 h n = 8. f Quantification of ectopic A08n postsynapses in C2da/C3da domain during development from 48 h AEL until 120 h AEL. Control 48 h n = 10, 72 h n = 8, 96 h n = 9, 120 h n = 10. UAS-TaoRNAi 48 h n = 10, 72 h n = 9, 96 h n = 10, 120 h n = 8. g Confocal images of hemisegments in control or with TaoRNAi expression in A08n neurons. Synaptic markers labeling C4da presynapses (magenta), A08n postsynapses (green), and anti-Fas3 immunolabeling C2da, C3da, and C4da sensory axons (blue) at 96 h AEL. Yellow arrowheads show ectopic A08n postsynapses within the C2da/C3da domain under TaoRNAi conditions. h XZ projections of each channel in g are shown. Scale bar = 5 µm. For all statistical tests: *P < 0.05, **P < 0.005, ***P < 0.001, **** P < 0.0001 ± SD, unpaired two-tailed t-test. For exact P values and statistics see Supplementary Data 1
