Fig. 2
From: Synaptotagmin 17 controls neurite outgrowth and synaptic physiology via distinct cellular pathways
Syt-17 mediates axon outgrowth. a Time-lapse DIC images of stage 3 neurons from wildtype (WT, top) and syt-17 knockout (KO, bottom) animals. Insets: growth cones at higher zoom. Scale bar indicates 10 μm. b Neurite outgrowth is normal at stage 2 (left, p > 0.1) but slowed at stage 3 (right, t19 = 3.095, p = 0.006, r2 = 0.335, meanwt = 9.04 ± 1.93 μm/h, meanko = 3.18 ± 0.46, Nwt = 10 and Nko = 11 neurons) in KOs. c Axonal morphology at 7 DIV. d Axon length was significantly reduced in syt-17 KO neurons (F2,68 = 6.65, p = 0.002, post-hoc of WT vs. KO: t41 = 4.397, p < 0.001, r2 = 0.32, meanwt = 5.23 ± 0.68 mm, meanko = 3.15 ± 0.31, Nwt = 19 and Nko = 24 neurons), and was rescued by exogenous expression of syt-17 (red; post-hoc of rescue vs. WT: p < 0.1, meanrescue = 5.03 ± 0.79, Nrescue = 17 neurons). Experiments were performed on 3–4 independent preparations of animals. Error bars indicate S.E.M.s
