Fig. 3
From: Maternal control of suspensor programmed cell death via gibberellin signaling
GA induces suspensor PCD via the NtCRF1-NtCYS pathway in vitro. a NtCRF1-GFP fluorescence in response to GA4 in four-celled proembryos. 0.05% ethyl alcohol as control. b NtCRF1-GFP fluorescence in the basal cells of two-celled proembryos during treatment with GA4 (or ABA) (n = 9). c NtCRF1-GFP in embryos derived from two-celled proembryo in cultured seeds after 72-h treatment with PAC. d Quantification of GA15, GA9, GA4, and GA51 in WT seeds at stages 1, 3, and 5 (pg per 1000 seeds, ± SE). e Cultured embryos stained with fluorescein diacetate (FDA), PI, and TUNEL. f Frequency of cultured embryos with PI-positive basal cells (n = 128–152). g Frequency of treated cultured embryos with TUNEL-positive basal cells (n = 106–126). h qRT-PCR analysis of NtCYS expression in 4-DAP seeds after treatment for 12-h with 100 μM ABA, PAC, or GA4. Data are the means ± SE of three independent experiments (Student’s t-test; ns, P > 0.05; **P < 0.01; ****P < 0.0001). Scale bars: 10 μm. The source data of the graphs are provided in the Source Data file
