Fig. 3
From: A family of anti-Bacteroidales peptide toxins wide-spread in the human gut microbiota
Analysis of the peptides produced from wild-type bacteria. a Alignment of the bacteriocin-like peptide of BvCL01/BvCL14 with that of BoCL02/BtCL15. The blue N-terminal sequence is the leader peptide predicted to be cleaved after the GG motif. Four cysteine residues in each peptide are highlighted in pink. b Alignment of the BoCL02/BtCL15 peptide with the class IIa bacteriocin pediocin from Pediococcus spp.. A conserved motif in class IIa bacteriocins of Gram-positive bacteria is underlined. c Western immunoblot analysis of the migration of reduced and non-reduced peptides from BvCL01 or BtCL15 probed with an antiserum generated to the synthesized peptides. d Results of LC–MS/MS analysis of the peptides from BvCL01/BvCL14 or BtCL15 showing the number of times each residue was detected by the number above. Yellow highlights indicate regions that were not detected by LC–MS/MS analysis. Source data are provided as a Source Data File
