Fig. 4
From: A family of anti-Bacteroidales peptide toxins wide-spread in the human gut microbiota
Analysis of killing activity by synthesized 3825 peptide. a Growth inhibition assays by the 3825 peptide (bacteroidetocin A) when 1 µg is applied in a 5 µl spot on top of an agar overlay containing the indicated strains. b Growth inhibition assays in broth culture when 2 µg of bacteroidetocin A (red) or water control (blue) is added to the indicated strain. All data represent the average of biological triplicates, ±SEM. c Colony forming unit (cfu/ml) quantification of viable bacteria following four hours in growth medium with bacteroidetocin A (red) compared to the starting cfu/ml (gray), or after four hours growth with added water (blue). Biological triplicates were performed for the growth assay with added bacteroidetocin A. Error bars represent standard error of the mean. d Agar overlay assays demonstrating the amount of bacteroidetocin A necessary to inhibit the growth of the overlay strains. e Titration of 3825 peptide in broth to determine the minimal inhibitory concentration for growth inhibition of B. thetaiotaomicron VPI 5482 and P. goldsteinii CL02. Source data are provided as a Source Data File
