Fig. 4

Angptl8 induces Per1 expression in vitro and in vivo. a qPCR analyses of the mRNA expression of core clock genes in mouse Hepa1c1c-7 hepatoma cells stimulated with either 40 nM Angptl8 or vehicle (PBS) for 2 h. n = 3, *P < 0.05 and **P < 0.01 Angptl8 vs. PBS, one-way ANOVA followed by Bonferroni’s post hoc test. b qPCR and Western blot analyses of Per1 expression in Hepa1c1c-7 cells stimulated with Angptl8 at indicated doses for 2 h. n = 3, **P < 0.01 Angptl8 vs. PBS, one-way ANOVA followed by Bonferroni’s post hoc test. c qPCR and Western blot analyses of Per1 expression in Hepa1c1c-7 cells stimulated with 40 nM Angptl8 for indicated times. n = 3, *P < 0.05 and **P < 0.01 vs.0 h, one-way ANOVA followed by Bonferroni’s posthoc test. d Reporter gene assays in Hepa1c1c-7 cells transfected with plasmids expressing Per1 promoter-luc (−2100 to +100 bp) for 36 h and then treated with 40 nM Angptl8, 50% horse serum, or indicated controls for another 2 h. n = 8, **P < 0.01 vs. No stimulus group, one-way ANOVA followed by Bonferroni’s post hoc test. e Per1 mRNA accumulation was assessed by CHX (cycloheximide) chase experiments. n = 3, **P < 0.01 Angptl8 vs. PBS, one-way ANOVA followed by Bonferroni’s post hoc test. f ICC analysis of Per1 intracellular localization in Hepa1c1c-7 cells stimulated with Angptl8 or PBS for 2 h. g qPCR and Western blot analyses of Per1 expression in livers of mice subjected to an acute injection of recombinant Angptl8 or vehicle (PBS) at ZT2. n = 5, *P < 0.05 Angptl8 vs. PBS at the same time point, one-way ANOVA followed by Bonferroni’s post hoc test. h qPCR and Western blot analyses of Per1 expression in the liver of mice subjected to an acute injection of Angptl8-neutralized antibody (50 μg/25 g, n = 8) or control IgG (n = 6) at ZT7. **P < 0.01 Anti-Angptl8 vs. control IgG at the same time point, one-way ANOVA followed by Bonferroni’s post hoc test. All data are presented as the means ± SD. Source data are provided as a Source Data file