Fig. 7
From: Angptl8 mediates food-driven resetting of hepatic circadian clock in mice
Angptl8 binds to its receptor PirB. a qPCR analyses of PirB mRNA expression in the liver of mice subjected to DD. n = 8. b, c qPCR and western blot analyses of PirB expression. Hepa1c1c-7 cells were stimulated with either 40 nM Angptl8 or PBS for 2 h. n = 3. d Co-IP assays in the mouse liver lysates. Immunoblots of precipitated proteins were performed by using indicated antibodies. e Co-IP assays in Hepa1c1c-7 cells cotransfected with plasmids encoding mouse Angptl8 and PirB. Immunoblots of precipitated proteins were performed by using indicated antibodies. f ICC analysis of colocalization of Angptl8 and PirB on the membrane of Hepa1c1c-7 cells treated with exogenous recombinant His-Angptl8 or vehicle (PBS) for 20 min. Source data are provided as a Source Data file
