Fig. 4
From: MpsAB is important for Staphylococcus aureus virulence and growth at atmospheric CO2 levels
mpsAB(C) complements an E. coli carbonic anhydrase mutant and vice versa. a E. coli carbonic anhydrase mutant (EDCM636) can be complemented with mpsAB and mpsABC using plasmid pRB473 under the control of its native promoter in atmospheric (left) and 5% CO2 (right). Clockwise from the top; EDCM636, EDCM636 pRB473 (empty plasmid), EDCM636 pRB473 mpsA, EDCM636 pRB473 mpsB, EDCM636 pRB473 mpsAB, and EDCM636 pRB473 mpsABC. b ∆mpsABC can be complemented with carbonic anhydrase, can from E. coli MG1655 using pTX30, a xylose-inducible promoter plasmid. Clockwise from the top; wild-type HG001, ∆mpsABC, ∆mpsABC pTX30 (empty plasmid), and ∆mpsABC pTX30 can. Plates were supplemented with xylose (+) or without xylose (−)
