Fig. 3 | Nature Communications

Fig. 3

From: HSP90 inhibitors stimulate DNAJB4 protein expression through a mechanism involving N6-methyladenosine

Fig. 3

HSP90 inhibitor-induced temporal changes in protein levels. Western blot images and the quantification data showing the alterations in expression levels of the HSP70, DNAJB4, METTL3, FTO, ALKBH5, and YTHDF3 in M14 cells at different time points following treatment with 100 nM ganetespib (a), AT13387 (b), or 17-DMAG (c). Shown are the ratios of expression of the indicated proteins over β-actin, and further normalized to the ratios obtained for the control cells without HSP90 inhibitor treatment. The data represent the mean ± S.D. of results from three independent experiments. The p-values referred to the comparison between 0 h and 24 h treatment and were calculated using unpaired, two-tailed Student’s t-test: #p > 0.05; *, 0.01 ≤ p < 0.05; **, 0.001 ≤ p < 0.01; ***, p < 0.001. Source data are provided as a Source Data file

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