Fig. 4
Modulation of DNAJB4 protein level by m6A writer and erasers. a Western blot for monitoring the expression levels of DNAJB4, Flag-tagged FTO, and ALKBH5, and endogenous FTO in M14 cells transfected with control plasmid or plasmids for the ectopic expression of Flag-FTO or ALKBH5 at different time intervals following treatment with 100 nM ganetespib. b Quantification data based on western blot analysis in (a). c Western blot for monitoring the expression levels of DNAJB4 in M14 cells treated with control non-targeting siRNA (siCtrl), siMETTL3–1 or siMETTL3–2 at different time points following treatment with 100 nM ganetespib. d Quantification data based on western blot analysis in (c). β-actin was employed as the loading control in (a) and (c). Shown in (b) and (d) are the ratios of expression of DNAJB4 protein over β-actin, and further normalized to the ratios obtained for the control cells without ganetespib treatment. The quantification data in (b) and (d) represent the mean ± S. D. of results from three independent experiments. The p-values referred to comparisons between control cells and cells with ectopic overexpression of the indicated genes (b), or between controls and siRNA-mediated knockdown of the indicated genes (d). The p-values were calculated using unpaired, two-tailed Student’s t-test: #p > 0.05; *, 0.01 ≤ p < 0.05; **, 0.001 ≤ p < 0.01; ***, p < 0.001. Source data are provided as a Source Data file
