Fig. 7
Heat shock induces elevated expression of DNAJB4 via m6A. Shown in (a), (c) and (e) are western blot images displaying the expression levels of DNAJB4 in M14 cells following heat shock treatment (HS, at 42.0 °C for 60 min), or the same cells after siRNA-mediated knockdown of METTL3 (siMETTL3–1) (a), ectopic overexpression of ALKBH5 (c), or the isogenic HEK293T cells with YTHDF1, YTHDF2, or YTHDF3 genes being knocked out by the CRSIPR-Cas9 genomic editing method (e). The relevant quantification data are presented in (b), (d) and (f). β-actin was employed as the loading control in (a), (c) and (e). Shown in (b) and (f) are the ratios of expression of DNAJB4 protein over β-actin, and in (d) these ratios were further normalized to the ratios obtained for the cells at t = 0 post heat shock. The quantification data in (b), (d) and (f) represent the mean ± S.D. of results from three independent experiments. The p-values referred to the comparison between control cells and siRNA-mediated knockdown (b) or ectopic expression (d) of the indicated genes, or HEK293T cells and the isogenic cells with YTHDF1/2/3 genes being individually ablated (f). The p-values were calculated based on unpaired, two-tailed Student’s t-test: *, 0.01 ≤ p < 0.05; **, 0.001 ≤ p < 0.01; ***, p < 0.001. Source data are provided as a Source Data file
