Fig. 5
From: Evolutionary modification of AGS protein contributes to formation of micromeres in sea urchins
Overexpression of AGS changes spindle orientation and anchoring. a AGS-GFP overexpression (AGS-OE; 1 μg/μl) changed overall embryo morphology at the 16-cell stage. Images were obtained by immunofluorescence with methanol fixation that diminishes the live GFP signal. The AGS-GFP signal was thus visualized by anti-GFP antibody. At M-phase, spindle orientation was randomized and many of the spindle poles were anchored to the nearby cortex (arrowheads in the magnified view). ~50% of embryos showed phenotype, n = 14. b, c AGS-OE caused ectopic recruitment of microtubules labeled by mCherry-EMTB to the cortex (arrows) in over 80% of embryos, n = 25. Images taken are from live embryos. Each experiment was performed at least three independent times. Scale bars = 20 μm
