Fig. 3
From: Expanding C–T base editing toolkit with diversified cytidine deaminases
Screening of base editing activities for CT-CBEs with/without the linker. a Organization of CT-CBEs. CT-CBEs were generated by fusing different cytidine deaminases to the C–terminus of nCas9. The 100 a.a.–length linker was inserted between nCas9 and cytidine deaminases, which was depleted in CT-CBEs (no linker). b Screening for the editing efficiencies of all CT-CBEs with the linker. c The linker was depleted in functional CT-CBEs (called CT-CBEs (no linker)) and their editing efficiencies were quantified and compared with the corresponding CT-CBEs. Two sgRNAs, sgRNA-A/B, and Sanger sequencing were used to evaluate the editing activities of CT-CBEs with/without the linker and C–T conversion frequencies at every cytosine located in regions 20bp upstream of PAM sites were quantified with EditR. Heat map showed the editing efficiencies for the critical C sites. Several C sites were not shown because of their low editing efficiencies for all CT-CBEs tested in this study. Source data are provided as a Source Data file
