Table 1 Features of different C–T base editors in this study
From: Expanding C–T base editing toolkit with diversified cytidine deaminases
Name | Deaminase | Editing window | Substrate preference C=edit site | Target Specificity (FAN/HEK4/EMX1) | ||
|---|---|---|---|---|---|---|
BE3 | rAPOBEC1 | 5–8 | TC/AC>CC>GC | 78% | 27% | 6% |
C-BE3 | rAPOBEC1 | 6,10 | TC/AC>CC>GC | 83% | 28% | 3% |
N-PmCDA1-BE | PmCDA1 | 1–8 | CA/CC>CG/CT | 8% | 15% | N.Sp. |
C-PmCDA1-BE | PmCDA1 | 1–6 | CA/CC>CG/CT | 29% | 18% | N.Sp. |
N-1-BE | PmCDA1L1_4 (1) | 9–13 | CA/CT>CG/CC AC/TC>CC/GC | 84% | 74% | 84% |
C-1-BE | PmCDA1L1_4 (1) | 10–13 | CA/CT>CG/CC AC/TC>CC/GC | 97% | N.Sp. | — |
N-2-BE | LpCDA1 (2) | 1–8 | N.S. | 42% | N.Sp. | N.Sp. |
C-2-BE | LpCDA1 (2) | −1,3–5 | N.S. | 85% | 31% | 14% |
N-4-BE | LjCDA1L1_4 (4) | −1–13 | CA/CC/CT>CG;AC>GC | N.Sp. | 7% | 40% |
N-7-BE | LjCDA1 (7) | 2–6 | CC/CT>CG | 81% | 61% | 68% |
N-8-BE | LpCDA1L1_1 (8) | 1–14 | CA>CG/CC AC/TC>CC/GC | 9% | 5% | 7% |
C-8-BE | LpCDA1L1_1 (8) | 9–14 | CA>CG/CC AC/TC>CC/GC | 55% | N.Sp. | 58% |
N-9-BE | LjCDA1L1_1 (9) | 1–15 | N.S. | N.Sp. | 6% | 1% |
N-10-BE | LpCDA1L1_3(10) | 1–13 | CC>CG;AC/TC>CC>GC | 85% | 24% | 47% |
N-12-BE | LjCDA1L2_1 (12) | 7–12 | N.S. | 81% | 95% | 42% |
N-13-BE | LpCDA1L1_4 (13) | 1–12 | CA/CC/CT>CG | 24% | 31% | 43% |
N-hAID-BE | hAID | 1–8 | CA/CC>CT AC/GC>CC/TC | 49% | 31% | N.Sp. |
C-hAID-BE | hAID | 3,5,7 | CA/CC>CT AC/GC>CC/TC | 75% | 86% | N.Sp. |
