Fig. 1
From: Engineering a microbial biosynthesis platform for de novo production of tropane alkaloids
Engineered pathway for de novo biosynthesis of tropane alkaloids in yeast. Color scheme for enzyme labels: orange, overexpressed yeast enzymes; green, plant enzymes; violet, bacterial enzymes; grey, spontaneous (non-enzymatic) step. Enzyme symbols: Arg2p, glutamate N-acetyltransferase; Car1p, arginase; AsADC, Avena sativa arginine decarboxylase; speB, agmatine ureohydrolase; Spe1p, ornithine decarboxylase; Fms1p, polyamine oxidase; AbPMT1, Atropa belladonna putrescine N-methyltransferase 1; DmMPO1ΔC-PTS1, Datura metel N-methylputrescine oxidase 1 with peroxisome targeting sequence 1 and truncated C-terminus; AbPYKS, A. belladonna pyrrolidine ketide synthase; AbCYP82M3, A. belladonna tropinone synthase; AtATR1, Arabidopsis thaliana NADP+-cytochrome P450 reductase; DsTR1, Datura stramonium tropinone reductase I; AtPAL1, A. thaliana phenylalanine ammonia-lyase; At4CL5, A. thaliana 4-coumarate-CoA ligase 5; EcCS, Erythroxylum coca cocaine synthase
