Fig. 5
From: A general approach for detecting expressed mutations in AML cells using single cell RNA-sequencing
GATA2R361C Subclonal expression signature. a t-SNE projection showing mutation-expressing cells in blue (GATA2R361C) and pink (TIMM17BL122fs). b Cells colored according to graph-based cluster assignment. c Heatmap of top 50 mutation-dependent genes, with bar graph showing mutant cell fraction in each AML cluster (labeled to the right of the heatmap). d Cells colored according to VIM expression (left), and scatterplot showing average VIM expression in each cluster as a function of the subclonal mutation fraction of each cluster (right). e t-SNE plot constructed from mutant cells, which are colored according to the mutation they contain: GATA2R361C, yellow; DNMT3AR882H, pink; FLT3-ITD, green; FLT3F612L, purple; NPM1W288FS; other somatic mutation(s), gray. f Mutant cells colored according to graph-based cluster. g Heatmap of top 25 subclonal mutation-dependent genes, with bar graph showing mutant cell fraction in each cluster (labeled to the right of the heatmap). Genes that are highly correlated with VIM in TCGA are indicated with blue dots. h Venn diagram indicating gene sets used to identify the VIM regulome
