Fig. 3
From: Transit amplifying cells coordinate mouse incisor mesenchymal stem cell activation
Lineage tracing MSCs in the incisor tooth. a Strategies of PDGFrβ Cre ERT2 and ROSA mT/mG mouse crossing and tamoxifen injection and sample harvesting are displayed. Illustration taken from https://www.somersault1824.com/science-illustrations/, which provides png images free to use and alter under the creative commons 4.0 license. b Representative mT/mG signal analysis at P7. Arrows mark clusters of odontoblasts that are EGFP positive. MTACs mesenchymal transit amplifying cells, NVB neurovascular bundle region. c, d Representative mT/mG signal analysis at P30. e–h Immunofluorescence analysis on the specific markers on P30 CD1 lower incisors. Nuclei were counterstained with DAPI. Yellow arrows mark clusters of odontoblasts that were EGFP positive. Light blue dotted lines indicate epithelial–mesenchymal junctions. Ods odontoblasts. Bars: b (left panel), c (left panel), and d: 80 μm; b (right panel), c (right panel), and e, g, h: 40 μm; f: 20 μm
