Fig. 4
IFNγ induces peptide processing and presentation in OPCs. a Immunocytochemical staining of primary C57BL/6 mouse OPCs which were cultured under PDGF conditions (20 ng/mL) or with IFNγ (10 ng/mL) for 12 h prior to addition of the MHC class I restricted MOG37–50 peptide (50 μg/mL) or OVA257–264 peptide (50 μg/mL) (scale bar = 50 μm). MCH class I restricted ovalbumin peptide presentation on OPCs was determined using PDGFRα and H2Kb-OVA257–264 antibodies. The right panel shows high magnification (25 μm) of IFNγ + OVA257-264 culture. b Time course experiments of WT and TAP−/− OPCs cultured with/without IFNγ and with/without OVA257–264 peptide. Treatment of IFNγ was completed for 12 h prior to the addition of no peptide (gray) or OVA257–264 peptide (blue) (c). MHC class I expression was determined from three stages of oligodendrocyte lineage cells either unstimulated or stimulated with IFNγ for 24 h. Oligodendrocyte lineage populations were defined by PDGFRα, A2B5, and O4 markers under proliferative or differentiating conditions (OPC vs. pOL mean diff = 30.9, 95% CI = −13.6–75.5, OPC vs. mOL mean diff = 54.1, 95% CI = 5.2–102.9)
