Fig. 2
Reclustering of the cluster c0. a Spectral tSNE plot of 1,231 cells from the cluster c0, colored according to gene expression, reveal 7 subclusters of cells (the subclusters c0-s0 to c0-s6). The number of cells (#) in each subcluster is as shown. b Similarity of the E15 subclusters c0-s0 to c0-s6 to the adult neurons n1 to n34, which include 24 ARC neurons highlighted in blue, was determined by calculating what % of genes specifically enriched in each subcluster belong to the top 200 genes enriched in each of the adult neurons n1 to n34. All seven subclusters showed significant similarity to at least one type of the adult neurons. c Spectral tSNE plot of 1,231 cells in the cluster c0, colored for the expression of representative key markers of ARC neurons, Npy/Otp/Sst/Agrp for AgrpSst-neurons, Th/Dlx1/Arx/Lhx1 for Th-neurons, Th/Dlx1/Ghrh for Ghrh-neurons, Pomc/Nhlh2 for Pomc-neurons, and Nhlh2/Sox14 for Kisspeptin-neurons. Violin plots for each gene are also shown
