Fig. 3

Developing ARC neurons from our scRNA-seq analysis of E15 ARC. Progenitors labeled by the transcription factor gene Nkx2-1 eventually give rise to different neuronal types in the adult ARC. Relative to the 24 ARC neuronal types defined by a recent RNA-seq analysis of adult ARC¹⁵, our scRNA-seq analysis of E15 ARC reveals several developing ARC neuronal types. Interestingly, cells in the subclusters c0-s0, c0-s1-s2, and c1-s3 appear to serve as common progenitors to multiple neuronal types in the adult ARC. Cells in the subcluster c0-s0 may represent developing neurons that later segregate to n4 Sst^Nts-neurons, n12 Agrp^Sst-neurons, and n23 Sst^Pthlh-neurons. Cells in the subcluster c0-s1-s2 may become n7 Arx^Nr5a2-neurons, n8 Th^Nfib-neurons, and n9 Th^Slc6a3-neurons, while cells in the subcluster c1-s3 may segregate to n14 Pomc^Ttr-neurons, n15 Pomc^Anxa2-neurons n21 Pomc^Glipr1-neurons. Cells in the subclusters c0-s3, c0-s4-s1, c0-s5, and c0-s6 may also give rise to n22 Tmem215-neurons, n14 Pomc^Ttr-neurons, n10 Ghrh-neurons, and n20 Kisspeptin-neurons, respectively