Fig. 3

MNS3 largely remains on punctate structures during Golgi disassembly. Fusion proteins were transiently expressed in N. benthamiana leaf epidermal cells and observed 2–3 dpi using confocal microscopy. a Confocal images showing the subcellular localization of MNS3-GFP before (0 h BFA) and after BFA addition (2 h BFA). Scale bars = 20 µm. b A representative cell that co-expresses MNS3-GFP (green) with STtmd-mRFP (magenta) and was treated with BFA for 2 h (upper panel) or additionally expresses GTP-locked SAR1 (SAR1-GTP, lower panel). Scale bars = 10 µm. c A representative cell that co-expresses MNS3-GFP (green) with the cis-Golgi golgin mRFP-AtCASP (magenta) and was treated with BFA for 2 h (upper panel) or additionally expresses SAR1-GTP (lower panel). Scale bars = 10 µm