Fig. 7 | Nature Communications

Fig. 7

From: High-throughput screen reveals sRNAs regulating crRNA biogenesis by targeting CRISPR leader to repress Rho termination

Fig. 7The alt text for this image may have been generated using AI.

PhrS-mediated anti-termination in CRISPR loci of multiple types CRISPR-Cas systems is a general phenomenon. a IntaRNA (Freiburg RNA tools) prediction of PhrS sRNA interaction with 5ʹUTR of CRISPR locus in multiple types CRISPR-Cas system. b CRISPR-lacZ activity for multiple types CRISPR-Cas system in WT or ΔphrS strain with or without BCM. c CRISPR-lacZ activity for multiple types CRISPR-Cas system in WT without PhrS overexpression, after PhrS overexpression. d Proposed model of PhrS-mediated regulation of CRISPR-Cas adaptive immunity. Bacterial small RNA PhrS inhibits the Rho-dependent termination to promote the expression of CRISPR loci, expanding function of sRNA in activating CRISPR-Cas adaptive immunity. Results are shown with mean ± SEM from three independent experiments. **P < 0.01, *P < 0.05, one-way ANOVA plus Tukey test

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