Fig. 8

Single-channel activity of the Ca²⁺ site mutants of LjCASTOR-2TM. a Sample traces of single-channel recordings of the Ca²⁺ site mutants from cell-attached patches. b Single-channel open probability of the Ca²⁺ site mutants of LjCASTOR-2TM; the number above the error bar indicates the number of measurements; *, p < 0.05. c Effect of Ca(I) site mutations on the cytosolic [Ca²⁺]-dependent activation of LjCASTOR-2TM. The recordings were obtained from the inside-out patches with 150 mM [Na⁺] in the pipette solution (extracellular). The bath solution (intracellular) contained 150 mM [K⁺] with various [Ca²⁺]. Data points are mean ± s.e.m. (n = 5 independent experiments)