Fig. 3
From: Blood–brain barrier permeable nano immunoconjugates induce local immune responses for glioma therapy
Increase of tumor infiltrating T cells revealed by flow cytometry. GL261 brain tumors were analyzed by the spectral flow cytometry (SONY Biotechnology) and tumor-associated T cells are presented as the cell counts in each treatment group. a CD3+ T cells; b CD3+CD4+ T helpers; c CD3+CD8+ T effectors; d CD3+CD4+Foxp3+ Tregs; e Ki67+ proliferating CD4+ cells; f Ki67+ proliferating CD8+ cells. Approximately 100,000 events/sample were recorded and analyzed by the SA3800 software (SONY Biotechnology). Data are mean ± SEM. *p < 0.05;. **p < 0.01; ***p < 0.001; ****p < 0.0001; N.S. = non-significant (one-way ANOVA with Sidak’s posttest). N = 6 per group
