Fig. 5
From: Blood–brain barrier permeable nano immunoconjugates induce local immune responses for glioma therapy
Increase of tumor macrophages, NK and NKT cells by flow cytometry. GL261 brain tumors were analyzed by the spectral flow cytometry (SONY Biotechnology) and various types of tumor-associated immune cells are presented as the cell counts in each treatment group. a Macrophages (MΦ): CD3-F4/80+; b M1 type MΦ: CD3-F4/80+iNOS+CD206-; c M2 type MΦ: CD3-F4/80+CD206+iNOS−; d Natural killer (NK) cells: CD3-NK1.1+; e Interferonγ+ NK cells; f NKT cells: CD3+NK1.1+. Approximately 100,000 events/sample are recorded and analyzed by the SA3800 software (SONY Biotechnology). Data are mean ± SEM. *p < 0.05;. **p < 0.01; ***p < 0.001; ****p < 0.0001; N.S. = non-significant (one-way ANOVA with Sidak’s posttest). N = 7 per group
