Fig. 6
From: Cell morphology and nucleoid dynamics in dividing Deinococcus radiodurans
Distribution and choreography of ter and oriC loci of chromosome 1. a Images of D. radiodurans strains GY15787 and GY15800 expressing HU-mCherry and a heterologous ParB fused to GFP (green foci), with multiple copies of its cognate parS sequence inserted nearby chromosome 1 ter (left) or oriC (right) loci, respectively (Table 1), and stained with the cell wall HADA dye (blue). For clarity, the HU-mCherry signal is not included in these images. Scale bar: 2 µm. b Number of ter and oriC foci detected per cell at the different phases of the cell cycle retrieved from 3D images. Data are represented as mean ± SD. c Weighted distribution density of ter (top) and oriC (bottom) foci at the different phases of the cell cycle. The red cross indicates the centre of the cell. b, c ter (N = 315; N > 37 for each phase) or oriC (N = 368; N > 36 for each phase) foci were analysed and data were retrieved from two independent experiments. d Examples of ter and oriC foci localization relative to the different nucleoid conformations of D. radiodurans, expressing HU-mCherry (red) and ParB-GFP (green), and stained with the cell wall dye HADA (blue). Scale bar: 1 µm. Source data are provided as a Source Data file
