Fig. 5

Sustained response to sequential combinatorial immunotherapy in rapidly evolving Her2-positive breast cancer. a Short-term experimental design for testing efficacy of indicated treatment schedule in MMTV-neu mice with spontaneous, advanced-stage tumors. b Kaplan–Meier progression free (without tumor volume increase) survival curves for different treatment and schedule as indicated in a (n = 6–8). P-value by log-rank (Mantel–Cox) test. c Long-term experimental design for testing efficacy of sequential combinatorial immunotargeted therapy in MMTV-neu mice with spontaneous, advanced-stage tumors. d Tumor volume curves of sequential combinatorial immunotargeted therapy (n = 9) as indicated in c. e Body weight measurements during treatment as performed in d. f The residual tumors after second-round sequential treatment as performed in d were transplanted to recipient syngeneic MMTV-neu mice (n = 6 for Ctrl and n = 9 for sequential treatment group). Relative tumor volumes of another round of sequential combinatorial immunotargeted therapy were shown