Fig. 1 | Nature Communications

Fig. 1

From: Replication stress triggers microsatellite destabilization and hypermutation leading to clonal expansion in vitro

Fig. 1The alternative text for this image may have been generated using AI.

MSI is induced as an alternative to CIN in MMR-deficient cells. a Msh2+/+ and Msh2−/− MEFs were cultivated under the Std-3T3 protocol to monitor the immortalization process. The graph shows mean cell numbers ± s.d. (n = 3 independent experiments with MEFs prepared from independent fetuses). b, c CIN-induction statuses were determined by flow cytometry (b) and G-band analysis (c). Green and red bars in b indicate diploidy and tetraploidy, respectively. d, e MSI statuses were determined by comparing fragment lengths at the indicated loci (d). The MSI status was determined in Msh2−/− MEFs at each stage (e). Red arrows indicate the shifted fragment peaks, i.e., MSI

Back to article page