Fig. 3

Replication stress induces hypermutation and MSI in Msh2^−/− MEFs. a–c Mutations were analyzed in Msh2^+/+ and Msh2^−/− MEFs at each cellular stage (a). Mutations detected in exons were categorized according to the indicated types (b, c). Mutations inducible with GT-mismatches are marked in yellow. InDel indicates In/Del mutations. d Mutations detected in exons of Msh2^+/+ and Msh2^−/− MEFs at IP1 and IP28 are indicated. The percentage of total reads in which each mutation was detected is shown. e The numbers of base substitutions observed (Obs) in exons of Msh2^−/− MEFs at IP1 and IP28 are indicated and compared with the numbers expected (Exp) based on replication errors. f, g In/Del mutations detected in Msh2^−/– MEFs were analyzed. Sequences around inserted/deleted bases in immortalized Msh2^−/− MEFs. The repeat numbers of insertions/deletions (g) and sequences around inserted/deleted bases in immortalized Msh2^−/− MEFs (h) were analyzed. A value of 0 indicates no repeats. h, In/Del mutations detected in exons of Msh2^−/− MEFs at IP28 are indicated. The percentage of total reads in which each mutation was detected is shown