Fig. 4
From: Structural basis of Cullin 2 RING E3 ligase regulation by the COP9 signalosome
Conformational response of CSN5/CSN6 to NEDD8. Differential HDX-MS of a Δ(CSN–CRL2~N8 - CSN) and b Δ(CSNWT–CRL2 - CSNWT). Regions exhibiting significant deuterium uptake differences in (i) CSN6, (ii) CSN5 and (iii) CSN5 active site are highlighted in red for destabilised and blue for stabilised areas. Regions without coverage or exhibit non-significant changes are in grey and white, respectively. c–d Deuteration profiles of CSN5 active site (as shown in a–b iii). Profiles of the c non-neddylated and d neddylated holocomplex (red) are compared with deuteration profiles of the isolated CSN (blue). The ion distribution of active site peptide is shown for c and d across reference 0 s, 15 s, 5 m and 30 m timepoints. The mass of the non-deuterated reference peptide is shown by the dotted line. A second dotted line for the holocomplexes (red) indicates the mass of the peptide at the 30 m timepoint. The relative deuterium changes of the active site peptide in c following binding of CRL2 is negligible, while in d, presence of CRL2~N8 leads to a significant increase in mass. Source Data are provided as a Source Data file
