Fig. 2

NPM1 is a new partner protein of hCINAP. a HEK293T cells harboring the Flag-empty vector or Flag-hCINAP were treated with or without IR (6 Gy) and then lysed and subjected to affinity purification using anti-Flag M2 affinity beads. The purified protein complex was analyzed by mass spectrometry. The major hits from mass spectrometry are shown in the table. b The interaction between endogenous hCINAP and NPM1 in HEK293T cells was confirmed by co-IP assays. Whole-cell lysates were immunoprecipitated with an antibody against NPM1 followed by immunoblotting using the indicated antibodies. c His-hCINAP and GST-NPM1 proteins were purified from E. coli, and in vitro pull-down analysis was performed using the GST protein as a negative control. d Mapping of the interaction domain of NPM1 with hCINAP. HEK293T cells expressing the Flag-hCINAP, HA-NPM1 WT, or NPM1 truncated mutants were subjected to the co-IP assays. A schematic of the different truncated mutants of NPM1 is shown. The source data can be found in Supplementary Data 1. Unprocessed scans of blots are provided in Supplementary Fig. 13