Fig. 1
From: EZHIP constrains Polycomb Repressive Complex 2 activity in germ cells
EZHIP interacts with PRC2 in gonads. a Volcano plot of EZH2 interactome from EZH2-Flag mice testis IP compared with WT. Core complex subunits are in red, in green the cofactors and in blue EZHIP, n = 3. b Volcano plot representation of EZHIP interactome after Flag-IP from HeLa-S3 overexpressing EZHIP compare with WT. Same color codes as in (a), n = 3. c Schematic representation of EZHIP protein sequence from Mus Musculus (upper part) and Homo Sapiens (middle part). Serine-rich region is colored in beige, and conserved amino acid stretch in green. The conserved sequence stretch is displayed as well as protein residues conservation between the two sequences in green (Sequence Homology determined using Genious software). d Ezhip and Ezh2 mRNA relative abundance normalized to Tbp in various mice tissues (mean, n = 2). e EZHIP and EZH2 IHC staining on human adult seminiferous tubules sections. Representative result, n ≥ 5. Scale bars, 50 μm. f EZHIP and EZH2 IHC staining on human adult ovaries sections, black arrows indicate the follicles. Representative result, n ≥ 5. Scale bars, 30 μm
