Fig. 6

Mature oocyte Ezhip−/− displays an altered epigenetic landscape. a Ezhip and Ezh2 expression (RPKM and FPKM) in germ cells during embryonic development and in oocytes isolated at different stages of follicular growth (PGC primordial germ cells, GC germ cells, NGO non-growing oocyte, GO growing oocyte phase I (8–14dpp) and phase II (15dpp), FGO fully grown oocytes; the data extracted from GSE94136 & GSE70116). b Single-cell RNA-seq Ezh2 and Ezhip expression data on early embryo developmental phases (oocyte, pronucleus, two cells, four cells, eight cells, and morula, data from GSE80810). c Quantification of H3K27me3 levels in P17 old females primordial and secondary follicles detected by immunofluorescence. Right, H3K27me3 intensities are normalized to DAPI. Left, representative image of secondary follicles stained with DAPI, H3K27me3, and merge, Ezhip + /− vs. Ezhip−/− . Scale bars, 30 μm. d Quantification of H3K27me3 levels by immunofluorescence in mouse surrounded nuclei (SN) GV oocytes. Top representative picture, bottom quantification. Scale bars, 5 μm. e Quantification of H3K27me3 levels by immunofluorescence in mature mouse MII oocytes. Top representative picture, bottom quantification. Scale bars, 5 μm. c–e Mean ± s.d., each dot represents a follicle, n indicated on the graph. Significance: unpaired, nonparametric test of Kologorov–Smirnov, ***P ≤ 0.001, **P ≤ 0.01, *P ≤ 0.05